Facultade de Fisioterapia

Levels of anti-CMV antibodies are modulated by the frequency and intensity of virus reactivations in kidney transplant patients

Iglesias-Escudero, Maria; Antonio Moro-Garcia, Marco; Marcos-Fernandez, Raquel; Garcia-Torre, Alejandra; Elena Alvarez-Arguelles, Marta; Luisa Suarez-Fernandez, Maria; Martínez Camblor, Pablo; Rodriguez, Minerva; Alonso-Arias, Rebeca
Abstract:
Anti-CMV (cytomegalovirus) antibody titers are related to immune alterations and increased risk of mortality. To test whether they represent a marker of infection history, we analyzed the effect of viral reactivations on the production of specific antibodies in kidney transplant patients. We quantified CMV-DNAemia and antibody titers in 58 kidney transplant patients before transplantation and during a follow-up of 315 days (standard deviation, SD: 134.5 days). In order to calculate the intensity of the infection, we plotted the follow-up time of the infection on the x-axis and the number of DNA-CMV copies on the y-axis and calculated the area under the curve (CMV-AUC). The degree of T-lymphocyte differentiation was analyzed with flow cytometry, the cells were labelled with different monoclonal antibodies in order to distinguish their differentiation state, from naive T-cells to senescent T-cells. Peak viremia was significantly higher in patients experiencing a primary infection (VI) compared to patients experiencing viral reactivation (VR). Our data indicate that the overall CMV viral load over the course of a primary infection is significantly higher than in a reactivation of a previously established infection. Whereas patients who experienced an episode of CMV reactivation during the course of our observation showed increased levels of CMV-specific antibodies, patients who did not experience CMV reactivation (WVR) showed a drop in CMV antibody levels that corresponds to an overall drop in antibody levels, probably due to the continuing immunosuppression after the renal transplant. We found a positive correlation between the CMV viremia over the course of the infection or reactivation and the CMV-specific antibody titers in the examined patients. We also observed a positive correlation between anti-CMV titers and T-cell differentiation. In conclusion, our data show that anti-CMV antibody titers are related to the course of CMV infection in kidney transplant patients.
Year:
2018
Type of Publication:
Article
Keywords:
Solid-Organ Transplantation; T-Cell Subsets; Cytomegalovirus-Infection; Cardiovascular-Disease; Norfolk Cohort; Immune-System; Older Women; Follow-Up; Mortality; People
Journal:
Plos One
Volume:
13
Number:
4 e0194789
Month:
April
Note:
Q1 15/64 h-index 2,766 (JCR2017)
DOI:
10.1371/journal.pone.0194789
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